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대한본초학회> 대한본초학회지(본초분과학회지)> 육계의 전립선암세포에서 YAP 활성 억제를 통한 전이 저해 효능 연구

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육계의 전립선암세포에서 YAP 활성 억제를 통한 전이 저해 효능 연구

Inhibitory effect of Cinnamomi Cortex extract on motility of prostate cancer cells through reducing YAP activity

정효원 ( Hyo Won Jung ) , 김옥현 ( Ok-hyeon Kim ) , 왕조유 ( Tsu Yu Wang ) , 김성은 ( Seong Eun Kim ) , 박용기 ( Yong-ki Park ) , 이현정 ( Hyun Jung Lee )
  • : 대한본초학회
  • : 대한본초학회지(본초분과학회지) 34권3호
  • : 연속간행물
  • : 2019년 05월
  • : 55-61(7pages)

DOI


목차

Ⅰ. 서 론
Ⅱ. 재료 및 방법
Ⅲ. 결과
Ⅳ. 고 찰
Ⅴ. 결 론
감사의 글
References

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초록 보기


						
Objectives : Recently, natural bioactive components catch a major attention for their potent anticarcinogenic activity. In this study, the inhibitory effect of Cinnamomi Cortex (CC) was examined in PC3 prostate cancer cells.
Methods : The toxicity of CC extract was evaluated with cell viability and cell morphology. The activity of Yes associated protein (YAP) was tested with qRT-PCR for the target gene expression such as CTGF and AMOTL2. Western blotting was performed for the evaluation of phospho-YAP level. For cell motility analysis, cellular motility was imaged by live imaging system for 6 hr. Successive images were used for the generation of movie file. Using this movie file, cellular migration was manually tracked and analyzed using time-lapse microscope and Fiji software.
Results : Cytotoxicity of CC extract was not detected at 500 ㎍/㎖ or below concentration. Although 500 ㎍/㎖ of CC extract reduced CTGF and AMOTL2 gene expression as YAP target genes, it was not statistically significant (CTGF expression P=0.0605, AMOTL2 expression P=0.4478). However, phosphorylated YAP was highly enhanced by CC extract treatment, when normalized with total YAP protein expression, suggesting YAP activation was inhibited. Finally prostate cancer cell motility was markedly reduced by 500 ㎍/㎖ of CC extract.
Conclusions : CC extract suppresses cancer cell motility and migration ability through inhibiting YAP activation without prostate cancer cell death, suggesting that this herb might be effective therapeutic drug for prostate cancer metastasis.

UCI(KEPA)

간행물정보

  • : 의약학분야  > 기초한의학
  • : KCI등재
  • :
  • : 격월
  • : 1229-1765
  • :
  • : 학술지
  • : 연속간행물
  • : 1986-2019
  • : 1557


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1아밀로이드 베타로 유발한 알츠하이머병 모델에서 신선초의 기억력 개선 효과

저자 : 이지혜 ( Jihye Lee ) , 김혜정 ( Hye-jeong Kim ) , 김동현 ( Dong-hyun Kim ) , 신범영 ( Bum Young Shin ) , 정지욱 ( Ji Wook Jung )

발행기관 : 대한본초학회 간행물 : 대한본초학회지(본초분과학회지) 34권 3호 발행 연도 : 2019 페이지 : pp. 1-7 (7 pages)

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Objectives : Amyloid β (Aβ) could induce cognitive deficits through oxidative stress, inflammation, and neuron death in Alzheimer's disease (AD). This study was investigated the effect of Angelica keiskei KOIDZUMI (AK) on memory in Aβ-induced an AD model.
Methods : AK was extracted uses 70% ethanol solvent. Total polyphenol and flavonoids content were obtained by the Folin-Ciocalteu and the Ethylene glycol colorimetric methods, respectively. The antioxidant activities were assessed through free radical scavenging assays using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazolin-6-sulfonic acid) (ABTS) methods. Intracerebroventrical (i.c.v) injection of Aβ 1-42 was used to induce AD in male ICR mice, followed by administrations of 5, 10 or 20 ㎎/㎏ AK on a daily. Animals were subjected to short and long term memory behavior in Y-maze and passive avoidance test.
Results : The total polyphenol and flavonoids contents of the AK extract were 88.73±6.36 ㎎ gallic acid equivalent/g, 84.21±5.04 ㎎ rutin equivalent/g, respectively. The assays of DPPH and ABTS revealed that AK extract in treated concentrations (31.25, 62.5, 125, 250, 500, 1000 ㎍/㎖) increased antioxidant activity in a dose-dependent manner. Oral administration of AK extract significantly reversed the Aβ 1-42-induced decreasing of the spontaneous alternation in the Y-maze test and Aβ 1-42-induced shorting of the step-through latency in the passive avoidance test.
Conclusions : The findings suggest that AK indicated the antioxidant protective effects against Aβ-induced memory deficits, and therefore a potential lead natural therapeutic drug or agent for AD.

2토종과 중국도입종간 꾸지뽕잎의 항알레르기작용 비교

저자 : 김광연 ( Kwang-yeon Kim ) , 하미애 ( Mi-ae Ha ) , 신용욱 ( Yong-wook Shin )

발행기관 : 대한본초학회 간행물 : 대한본초학회지(본초분과학회지) 34권 3호 발행 연도 : 2019 페이지 : pp. 9-17 (9 pages)

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Objectives : This study was performed to compare the effect of two Cudrania tricuspidata cultivars; Sancheong native (CT) and varieties in china(SCT) on immediate hypersensitivity of the anaphylactic type and Ova-induced allergic asthma mouse model by calculating serum cytokines and IgE.
Methods : We investigated the free radical scavanging effect and quantify total phenol contents and total flavonoids of two Cudrania tricuspidata cultivars; Sancheong native(CT) and varieties in china(SCT). The sample was extracted by 80% EtOH. To induce the allergic asthma, in the control group and the CT group, mice of each group were sensitized intraperitoneally with ovalbumin (OVA) solution at the 1st, the 7th and the 14th day. After then, sensitization was performed by aerosol allergen challenges with 1% OVA solution intratracheally at the 21th, the 23th, 25th and the 27th day. At the 29th day, the mice were killed and the changes of interferon-γ, interleukin-4, 5 and 10, total IgE and OVA-specific IgE in serum were checked.
Results : CT inhibited compound 48/80-induced systemic anaphylaxis 90% with a dose of 100 ㎎/㎏ body weight at 1 hr before injection of compound 48/80. In the allergic asthma mouse model, IFN-γ was did not increased in the CT and SCT group than that in the control group. IL-4, IL-5, the total IgE and OVA-specific IgE were decreased in the CT group as compared with the control group and these results were statistically significant.
Conclusions : Considering the above experimental results, this study showed that Sancheong native cultivar could reduce the allergic reaction.

3Stereoscope를 이용한 미세종자류 한약재 외부형태 감별연구(제3보(報)) - 사원자(沙苑子)와 황기자(黃芪子), 정력자(葶藶子)-

저자 : 김영식 ( Young-sik Kim ) , 주영승 ( Young-sung Ju )

발행기관 : 대한본초학회 간행물 : 대한본초학회지(본초분과학회지) 34권 3호 발행 연도 : 2019 페이지 : pp. 19-30 (12 pages)

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Objectives : This study is to propose the identification keys based on stereoscopic examination of 8 seed herbs in 2 categories (Phyllolobobii Semen (PS) with 4 Astragali Semen (AS), and 2 Lepidii seu Descurainiae Semen (LDS) with 1 Drabae Semen (DS)) which have difficulties in discrimination with visual observation.
Methods : We reviewed the description of original plants and their medicinal parts from the literature. The original plants were collected, identified, confirmed as specimens, and compared to the samples distributed in the market. The first identification was made by visual observation, and insufficient points were supplemented by stereoscopic observation. Identification criteria were set by considering morphological characteristics of authentic herbs, percentage of adulterants, and distinction between authentic herbs and adulterants.
Results : The original plants of PS and AS could be distinguished by upright or lying form of stem, color of flowers, number of leaflets, and presence of hair of fruits. LDS and DS could be distinguished by leaf arrangement on stem: radical or cauline, whole plants size, leaf division, color of flowers, and shape of fruits. The herbal medicines of PS and AS could be distinguished by seed surface pattern, size, and hardness. LDS and DS could be distinguished by size, shape, viscosity when chewed, and degree of mucous layer formation when soaked in water.
Conclusions : This study suggests the identification keys of original plants and herbal medicines. Especially, since fine seed herbs are difficult to distinguish by visual observation, the stereoscope should be applied to the discrimination.

4산수유, 보골지 복합추출물의 Sprague-Dawley rat를 이용한 단회경구투여 독성시험

저자 : 진보람 ( Bo-ram Jin ) , 강현애 ( Hyun-ae Kang ) , Divina C. Cominguez , 김명석 ( Myoung-seok Kim ) , 이광호 ( Kwang-ho Lee ) , 윤일주 ( Il-joo Yoon ) , 안효진 ( Hyo-jin An )

발행기관 : 대한본초학회 간행물 : 대한본초학회지(본초분과학회지) 34권 3호 발행 연도 : 2019 페이지 : pp. 31-36 (6 pages)

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Objectives : In this animal study, we performed the single oral dose toxicity test of Standardized Cornus officinalis Sieb. et Zucc. and Psoralea corylifolia L. 30% ethanol extract (SCP) in Sprague-Dawley (SD) rats owing to aims for verifying approximate lethal dose (ALD).
Methods : According to OECD guidelines for the testing of chemicals section 4 health effects test No. 420 acute oral toxicity study - fixed dose procedure (17 December 2001), single oral dose toxicity test was performed. Animals were divided into two groups: Group 1, vehicle-treated rats (Control); Group 2, SCP 5000 ㎎/㎏ treated rats. SCP is composed of two medicinal herbs: Cornus officinalis Sieb. et Zucc. (650 g) and Psoralea corylifolia L. (350 g) in 30% ethanol. SCP was once orally administered to female and male SD rats at dose levels of 5000 ㎎/㎏. Animals were monitored on the mortality, clinical signs, body weight changes and necropsy findings for 14 days.
Results : After single oral treatment of SCP, we could not find any mortality up to 5000 ㎎/㎏. Compared with the control group, there were also no significant differences in clinical sign, weight change, weight gain and gross abnormalities in SCP 5000 ㎎/㎏-treated group.
Conclusions : Taken together, these results suggest that the ALD of SCP in both female and male SD rats were considered as over 5000 ㎎/㎏. Results from this study provide scientific evidence for the safety of SCP.

5한약 저온숙성비누가 여드름 피부에 미치는 영향

저자 : 최상락 ( Sang Rak Choi ) , 서부일 ( Bu Il Seo ) , 구진숙 ( Jin Suk Koo )

발행기관 : 대한본초학회 간행물 : 대한본초학회지(본초분과학회지) 34권 3호 발행 연도 : 2019 페이지 : pp. 37-44 (8 pages)

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Objectives : Acne is a common disease that affects more than 70% of adolescents.
Acne patients have a poor quality of life compared to patients with other skin diseases. We tried to demonstrate the effectiveness of acne treatment using cleansing soap which is easily used in daily life.
Methods : We selected 20 students with acne symptoms on their facial skin. We made herbal Cp (Cold process) soaps using Seosiokyongsan, Kyungohkgo, Hwangryunhaedoktang and Baeksoooh and distributed them to experiment participants. We let them wash their face in the morning and evening for 6 weeks using herbal Cp soap. Prior to the experiment, their skin condition was checked and assessed using A-ONE Smart One-Click Automatic Facial Diagnosis System three times at 3-week intervals. Acne status was classified into 6 stages according to KAGS and acne status was also measured 3 times in total. After the experiment, the changes of skin were analyzed through facial analysis test.
Results : Based on the KAGS classification, the condition of acne has improved as a whole. The state of moisture was gradually increased and the state of skin oil was significantly decreased after 6 weeks of using soap compared to before using soap.
Conclusions : Cp soaps made from four kinds of herbal medicine are believed to improve the condition of acne by increasing the moisture of the facial skin and decreasing the skin oil content.

6황기가 6-Propyl-2-thiouracil(PTU)로 유발된 rat의 갑상선기능저하증에 미치는 영향

저자 : 이지혜 ( Ji Hye Lee ) , 구진숙 ( Jin Suk Koo ) , 노성수 ( Seong Soo Roh ) , 박지하 ( Ji Ha Park ) , 서부일 ( Bu Il Seo )

발행기관 : 대한본초학회 간행물 : 대한본초학회지(본초분과학회지) 34권 3호 발행 연도 : 2019 페이지 : pp. 45-53 (9 pages)

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Objectives : In present study, we investigated a therapeutic effect of Astragali Radix on hypothyroidism rat model induced by 6-Propyl-2-thiouracil (PTU).
Methods : Six-week-old male Sprague-Dawley rats were divided into five groups : Group one included the normal mice. Group two was administrated PTU. Group three and four were administrated the aqueous extract of Astragali Radix 150 and 300 ㎎/㎏ before start of PTU treatment. Group five (Positive control) was administrated with levothyroxine 0.5 ㎎/㎏. During this moment the body weight, liver H2O2 and catalase (CAT) amount, serum thyroid hormone, serum asparte aminotransferase (AST) and alanine aminotransferase (ALT), gland weights were measured with histopathological changes of thyroid glands. These results were compared with levothyroxine 0.5 ㎎/㎏ treated rats.
Results : The PTU treatment lead to marked decreases of body weight, level of thyroid hormone in serum and liver CAT activation. Also, PTU treatment increased thyroid gland weight, thyroid gland hormone TSH, liver H2O2 amount and level of AST in serum. On the other hands, the administration of Astragali Radix extract increased body weight gains and ameliorated histopathological changes of thyroid such as hyperplasia of follicular cells with of follicular colloid contents and sizes. In addition, the administration of Astragali Radix extract increased level of T4 in serum, CAT activation in liver. Moreover, the administration of Astragali Radix extract decreased levels of TSH and AST in serum and H2O2 amount in liver
Conclusions : This study suggests that Astragali Radix extract has therapeutic effects on hypothyroidism via promoting thyroid hormone production.

7육계의 전립선암세포에서 YAP 활성 억제를 통한 전이 저해 효능 연구

저자 : 정효원 ( Hyo Won Jung ) , 김옥현 ( Ok-hyeon Kim ) , 왕조유 ( Tsu Yu Wang ) , 김성은 ( Seong Eun Kim ) , 박용기 ( Yong-ki Park ) , 이현정 ( Hyun Jung Lee )

발행기관 : 대한본초학회 간행물 : 대한본초학회지(본초분과학회지) 34권 3호 발행 연도 : 2019 페이지 : pp. 55-61 (7 pages)

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Objectives : Recently, natural bioactive components catch a major attention for their potent anticarcinogenic activity. In this study, the inhibitory effect of Cinnamomi Cortex (CC) was examined in PC3 prostate cancer cells.
Methods : The toxicity of CC extract was evaluated with cell viability and cell morphology. The activity of Yes associated protein (YAP) was tested with qRT-PCR for the target gene expression such as CTGF and AMOTL2. Western blotting was performed for the evaluation of phospho-YAP level. For cell motility analysis, cellular motility was imaged by live imaging system for 6 hr. Successive images were used for the generation of movie file. Using this movie file, cellular migration was manually tracked and analyzed using time-lapse microscope and Fiji software.
Results : Cytotoxicity of CC extract was not detected at 500 ㎍/㎖ or below concentration. Although 500 ㎍/㎖ of CC extract reduced CTGF and AMOTL2 gene expression as YAP target genes, it was not statistically significant (CTGF expression P=0.0605, AMOTL2 expression P=0.4478). However, phosphorylated YAP was highly enhanced by CC extract treatment, when normalized with total YAP protein expression, suggesting YAP activation was inhibited. Finally prostate cancer cell motility was markedly reduced by 500 ㎍/㎖ of CC extract.
Conclusions : CC extract suppresses cancer cell motility and migration ability through inhibiting YAP activation without prostate cancer cell death, suggesting that this herb might be effective therapeutic drug for prostate cancer metastasis.

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