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한국응용약물학회> Biomolecules & Therapeutics(구 응용약물학회지)> 원보 : 산화적 스트레스 및 항산화제가 항산화효소 활성에 미치는 영향

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원보 : 산화적 스트레스 및 항산화제가 항산화효소 활성에 미치는 영향

Original articles : Alterations of Antioxidant Enzymes in Response to Oxidative Stress and Antioxidants

김안근(An Keun Kim) , 김지현(Ji Hyun Kim)
  • : 한국응용약물학회
  • : Biomolecules & Therapeutics(구 응용약물학회지) 9권4호
  • : 연속간행물
  • : 2001년 12월
  • : 249-257(9pages)

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N/A
The effect of oxidative stress on the alterations of different antioxidant enzyme activities was investigated in human skin melanoma cell line (SK-MEL-2). Oxidative stress was induced by the exposure to hydrogen peroxide (H_2O_2). SK-MEL-2 cells were treated with antioxidants such as vitamin E and selenomethionine in combination with H_2O_2. SK-MEL-2 cells were exposed to various concentrations of H_2O_2 and measured the time course of changes in cell viability and antioxidant enzyme activities for 24 hr. Oxidative stress was induced by the exposure to 2.5mM hydrogen peroxide (H_2O_2) resulted in declining significantly for 24 hr. GPX and CAT activities peaked at 3 hr and returned to control levels by 24 hr. On the contrary, SOD activity was inactive before 6 hr but recovered at 24 hr. In case vitamin E (Vit E) and selenomethionine (SeMet) were used at nontoxic concentrations (25μM Vit E/500μM Se-Met) to oxidative stress was induced by the exposure to hydrogen peroxide (H_2O_2) led to a 3- and 5-fold increase on the viability comparing to control and caused an increase in GPX activity respectively.

UCI(KEPA)

I410-ECN-0102-2009-510-004671859

간행물정보

  • : 의약학분야  > 약화학
  • : KCI등재
  • : SCI,SCOPUS
  • : 격월
  • : 1976-9148
  • : 2005-4483
  • : 학술지
  • : 연속간행물
  • : 1993-2020
  • : 1647


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This study was attempted to investigate the effects of cromakalim (CRK), K^+ channel opener, and glibenclamide (GLY), K^+ channel blocker, on intestinal function of rabbit. CRK supressed the tension and spontaneous movement of intestinal strips. CRK enhanced the tension and spontaneous movement of strips induced by acetylcholine. Also the inhibiting effect of dopamine was potentiated by CRK. GLY augmented the tension, but did not affect the spontaneous movement of strips. GLY inhibited tension and spontaneous movements in intestinal strips induced by acetylcholine, GLY blocked the dopamine-induced attenuation of tension, but not the decrease of spontaneous movements in intestinal strips. The present studies suggest that K^+ channel opening suppresses intestinal movements, whereas it`s blockade enhances intestinal movements in rabbit.

2원보 : 홍화자 추출물과 키토산 병용처리에 의한 경조직 재생촉진 효과

저자 : 정세영(Se Young Choung) , 박준봉(Joon Bong Park) , 박건구(Kun Koo Park) , 권영혁(Young Hyuk Kwon) , 김성진(Sung Jin Kim)

발행기관 : 한국응용약물학회 간행물 : Biomolecules & Therapeutics(구 응용약물학회지) 9권 4호 발행 연도 : 2001 페이지 : pp. 244-248 (5 pages)

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This study was performed to investigate therapeutic effects of Carthami Semen, Paeoniae Radix extracts and chitosan on the growth and differentiation of human periodontal ligament cell. We found that co-treatment of methanol extracts of Carthami Semen and chitosan significantly increased the growth of human periodontal ligament cell. However, the sigle treatment groups of the extracts showed only 20∼30% of the growth increase. Alkaline phosphase activity, one of differentiation markers, was increased approximately 1.5-fold by co-treatment of methanol extract of Carthami Semen and chitosan and calcified nodule formation was also increased at the similar levels as the alkaline phosphatase. But the single treatment groups showed only 20∼30% increases. These results suggest that Carthami Semen and chitosan co-treatment can be used efficiently for periodontium regeneration.

3원보 : 산화적 스트레스 및 항산화제가 항산화효소 활성에 미치는 영향

저자 : 김안근(An Keun Kim) , 김지현(Ji Hyun Kim)

발행기관 : 한국응용약물학회 간행물 : Biomolecules & Therapeutics(구 응용약물학회지) 9권 4호 발행 연도 : 2001 페이지 : pp. 249-257 (9 pages)

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The effect of oxidative stress on the alterations of different antioxidant enzyme activities was investigated in human skin melanoma cell line (SK-MEL-2). Oxidative stress was induced by the exposure to hydrogen peroxide (H_2O_2). SK-MEL-2 cells were treated with antioxidants such as vitamin E and selenomethionine in combination with H_2O_2. SK-MEL-2 cells were exposed to various concentrations of H_2O_2 and measured the time course of changes in cell viability and antioxidant enzyme activities for 24 hr. Oxidative stress was induced by the exposure to 2.5mM hydrogen peroxide (H_2O_2) resulted in declining significantly for 24 hr. GPX and CAT activities peaked at 3 hr and returned to control levels by 24 hr. On the contrary, SOD activity was inactive before 6 hr but recovered at 24 hr. In case vitamin E (Vit E) and selenomethionine (SeMet) were used at nontoxic concentrations (25μM Vit E/500μM Se-Met) to oxidative stress was induced by the exposure to hydrogen peroxide (H_2O_2) led to a 3- and 5-fold increase on the viability comparing to control and caused an increase in GPX activity respectively.

4원보 : Endotoxin 에 의한 혈전증에 미치는 홍화자의 효과

저자 : 승금란(Keum Ran Seung) , 정기화(Ki Hwa Jung)

발행기관 : 한국응용약물학회 간행물 : Biomolecules & Therapeutics(구 응용약물학회지) 9권 4호 발행 연도 : 2001 페이지 : pp. 258-262 (5 pages)

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In the advanced age, cardiovascular disease is more serious than any other disease. Especially, the thrombus causes the serious disease like apoplexia, cerebri and myocardial infarction. Thrombosis is caused by the injury of endothelium and the alterations in normal blood flow. To investigate activities of Carthamus tinctorius L. Semen butanol fraction for blood coagulation system, endotoxin (4000EU/㎏) was injected (i..v) to rats at 1hr after administration of Carthamus tinctorius L. Semen butanol fraction (500㎎/㎏, p.o.). Carthamus tinctorius L. Semen butanol fraction was found to have antiplatelet activity in vitro. In vivo it showed a delay of blood clotting time, and prothrombin time, and reduction of fibrinogen and FDP. It also increased SOD acitivity, and decreased MDA content. These results suggest that the antithrombosis effect of Carthamus tinctorius L. Semen butanol fraction results from suppressive activity for a blood coagulation system and antioxidative activity.

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In this study, we tried to investigate whether poly-L-arginine (PLA) (MW 10,800) significantly affect mucin release from cultured hamster airway goblet cells and the mucosubstances of hypersecretory airway goblet cells of rats. Confluent primary hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled with ^3H-glucosamine for 24 hr and chased for 30 min in the presence of varying concentrations of PLA to assess the effects on ^3H-mucin release. Possible cytotoxicities of PLA were assessed by measuring both Lactate Dehydrogenase (LDH) release and by checking the possible changes on the morphology of HTSE cells during treatment. For in vivo experiment, hyperplasia of rat airway goblet cells and increase in intraepithelial mucosubstances were induced by exposing rats to SO_2 for 3 weeks and varying concentrations of PLA were administered inhalationally to assess the effects on the mucosubstances of airway goblet cells of rats. The results were as follows : (1) PLA significantly inhibited mucin release from cultured HTSE cells in a dosedependent manner; (2) there was no significant release of LDH and no significant change on the morphology of cultured HTSE cells during treatment; (3) PLA also affected the intraepithelial mucosubstances of hypersecretory rats and restored them to the levels of control animals. We conclude that PLA inhibit mucin release from airway goblet cells without significant cytotoxicity and possibly normalize the hypersecretion of airway mucosubstances in vivo. This finding suggests that PLA might function as an airway mucoregulative agent.

6원보 : Vanadium yeast 결합체의 항당뇨 효과

저자 : 박승희(Seung Hee Park) , 정규혁(Kyu Hyuck Chung)

발행기관 : 한국응용약물학회 간행물 : Biomolecules & Therapeutics(구 응용약물학회지) 9권 4호 발행 연도 : 2001 페이지 : pp. 270-276 (7 pages)

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Vanadium yeast was prepared by uptaking vanadate in yeast cells. The growth rate of yeast cells was enhanced by 1∼5% glucose. While the growth rate of yeast cells was not significantly affected by YEPD containing less than 1mM vanadate, it was completely inhibited by 2.5 mM vanadate. Vanadium uptake in yeast cells was increased with increasing vanadate concentration in growth medium. Vanadate (Ⅴ) was reduced to vanadyl (Ⅳ) in yeast cells associating with macromolecular compounds in cells. Oral administration of vanadium yeast significantly reduced blood glucose levels of streptozotocin treated rats same as vanadate. Vanadate and vanadium yeast similarly increased glucose oxidation in isolated adipocytes. Therefore, it was suggested that vanadium yeast could have an antidiabetic activity potency similar to that of vanadate.

7원보 : 1 형과 2 형 당뇨모델 흰쥐에서 Chromium Picolinate 의 당내성과 인슐린 감수성에 대한 영향

저자 : 신현진(Hyun Jin Shin) , 홍정희(Jeong Hee Hong) , 고현철(Hyun Chul Koh) , 신인철(In Chul Chin) , 최호순(Ho Soon Choi) , 김태화(Tae Hwa Kim) , 김동선(Dong Sun Kim) , 엄애선(Ae Son Om) , 강주섭(Ju Seop Kang)

발행기관 : 한국응용약물학회 간행물 : Biomolecules & Therapeutics(구 응용약물학회지) 9권 4호 발행 연도 : 2001 페이지 : pp. 277-281 (5 pages)

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Chromium is an essential nutrient and participates in glucose and lipid metabolism in human beings and animals. The present study was conducted to assess the effects of chromium picolinate (Cr-pic) on glucose tolerance and insulin sensitivity in type Ⅰ and Ⅱ diabetic rats. The experimental groups were type Ⅰ diabetic (streptozotocin-induced: 40㎎/㎏, i.p.) and type Ⅱdiabetic (Goto-Kakizaki rats) models. Each group was subdivided into control, low-dose and high-dose of Cr-pic treated groups. The Cr-pic was orally administered with Cr-pic (100 mg/kg for low dose group and 200㎎/㎏ for high dose group) for 4 weeks. And then we performed intraperitoneal glucose tolerance test (IPGTT) and insulin sensitivity test (ITT). The glucose tolerance test was carried out by injection of glucose (2 g/㎏, i.p.). The peripheral insulin sensitivity test was conducted by injection of insulin (5 units/㎏, s.c.) and glucose. We performed determining of blood glucose concentration at 0, 10, 30, 60, 90, and 120 min using automated glucose analyzer. The plasma insulin concentration was determined by rat insulin EIA kit. Administration of Cr-pic improved weight gain in all groups with higher significant in the low-dose group. There was no significance between the control and the Cr-pic treated groups in the area under the blood glucose curve and serum insulin concentration plots of IPGTT and peripheral ITT in type Ⅰ diabetic rats. But Cr-pic treated groups showed significantly lower levels of the area under the blood glucose curve during IPGTT and ITT and the high-dose group showed less effects compared with the low-dose group in the type Ⅱ diabetic rats. The plasma insulin concentration of both diabetic groups was not influenced by Cr-pic supplementation. We can conclude that chromium picolinate may improve the endogenous and exogenous insulin action and peripheral insulin sensitivity in type Ⅱ diabetic rats.

8Original articles : Comparison of the Antihistaminic Activity Between Cetirizine Enantiomers

저자 : Hae Young Park Choo , Sun Ok Choi , Seok Ho Lee

발행기관 : 한국응용약물학회 간행물 : Biomolecules & Therapeutics(구 응용약물학회지) 9권 4호 발행 연도 : 2001 페이지 : pp. 282-284 (3 pages)

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The antiallergic drug, cetirizine, inhibits the histamine release from a rat basophilic leukemia (RBL-2H3) cell line, which is frequently used as a mast cell model. By investigating inhibitory activities of (+)- and (-)-cetirizine in RBL-2H3 cells on the histamine release, we aimed to evaluate the effect of their structual characteristics on the antihistamine activity. The study on RBL-2H3 cell has clearly demonstrated that the (-)-cetirizine is significantly more potent than the (+)- or the racemic cetirizine, although there was no difference in pharmacokinetics between (+)- and (-)-cetirizine in rats.

9원보 : 아세틸 - 엘 - 카르니틴 정제의 생물학적 동등성 평가

저자 : 박경미(Kyung Mi Park) , 이미경(Mi Kyung Lee) , 신지영(Jee Young Shin) , 우종수(Jong Soo Woo) , 임수정(Soo Jeong Lim) , 임윤영(Yoon Young Lim) , 김종국(Chong Kook Kim)

발행기관 : 한국응용약물학회 간행물 : Biomolecules & Therapeutics(구 응용약물학회지) 9권 4호 발행 연도 : 2001 페이지 : pp. 285-290 (6 pages)

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Bioequivalence of two acetyl-l-carnitine tablets, test product (Carnitile tablet: Hanmi Pharm. Co., Ltd.) and reference product (Nicetile^? tablet: Dong-A Pharm. Co., Ltd.), was evaluated according to the guidelines of Korea Food and Drug Administration (KFDA). Twenty-six healthy volunteers were divided randomly into two groups and administered the drug orally at the dose of 500㎎ as acetyl-l-carnitine in a 2×2 crossover study. Blood samples were taken at predetermined time intervals for 12 hours and the plasma concentration of acetyl-l-carnitine was determined using HPLC by derivatization with p-bromophenacyl bromide. The pharmacokinetic parameters (AUC_(0-12h), C_max, and T_max.) were calculated and ANOVA was utilized for the statistical analysis of parameters. The apparent differences of these parameters between two drugs were less than 20% (i.e., 1.26, -5.08 and 8.59% for AUC_(0-12h), C_max, and T_max, respectively). The powers (1-β) for AUC_(0-12h), C_max and Tmax were over 0.9. Minimal detectable difference (Δ) at α=0.05, I-β=0.8 were less than 20% (i.e., 7.31, 14.88 and 11.77% for AUC_(0-12h), C_max, and T_max, respectively). The confidence intervals (δ) for these parameters were also within ± 20% (i.e., -3.03≤δ≤5.54, -13.80≤δ≤3.64 and 1.69≤δ≤15.48 for AUC_(0-12h), C_max, and T_max, respectively). These results satisfied the criteria of KFDA guideline for bioequivalence, indicating Carnitile bioequivalent to Nicetile^?

10원보 : 트리플루살 캅셀의 생물학적 동등성 평가

저자 : 박경미(Kyung Mi Park) , 이미경(Mi Kyung Lee) , 임수정(Soo Jeong Lim) , 김종국(Chong Kook Kim) , 박정숙(Jeong Sook Park) , 김진기(Jin Ki Kim) , 최성희(Sung Hi Choi) , 민경아(Kyung Ah Min)

발행기관 : 한국응용약물학회 간행물 : Biomolecules & Therapeutics(구 응용약물학회지) 9권 4호 발행 연도 : 2001 페이지 : pp. 291-297 (7 pages)

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The bioequivalence of two triflusal products was evaluated with 20 healthy volunteers following single oral dose according to the guidelines of Korea Food and Drug Administration (KFDA). Trisal^? capsule (Whanin Pharm. Corp., Korea) and Disgren^? capsule (Myung-In Pharm. Corp., Korea) were used as test product and reference product, respectively. Both products contain 300㎎ of trifusal. One capsule of test product or reference product was orally administered to the volunteers, respectively, by randomized two period crossover study (2×2 Latin square method). Blood samples were taken at predetermined time intervals for 4 hours and the determination of trifusal was accomplished using semi-microbore HPLC equipped with automated column switching system. The analytical method with HPLC was validated according to the Bioanalytic Method Validation guideline by FDA prior to determining the plasma samples. The pharmacokinetic parameters (AUC_(0-4h), C_max, and T_max) were calculated and ANOVA test was utilized for statistical analysis of parameters. As a result of the assay validation, the limit of quantification of trifusal in human plasma by current assay procedure was 50 ng/ml using 500 ㎕ of plasma. The accuracy of the assay was from 97.76% to 116.51% while the intra-day and inter-day coefficient of variation of the same concentration range was less than 15%. Average drug concentration at the designated time intervals and pharmacokinetic parameters calculated were not significantly different between two products (p>0.05). The difference of mean AUC_(0→4hr), C_max, and T_max between the two products (2.92, 4.39, and -2.44%, respectively) were less than 20%. The power (1-β) and treatment difference (Δ) for AUC_(0→4hr) and C_max were more than 0.8 and less than 0.2, respectively. Although the power for T_max was under 0.8, T_max, of the two products was not significantly different from each other (p>0.05). These results satisfied the criteria of KFDA guideline for bioequivalence, indicating the two products of triflusal were bioequivalent.

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